Part:BBa_K3956010:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3956010

The scl2 construct was expressed with the cpTEF-6 promoter and PRM9 terminator in a pRS4116 backbone. Although we were able to verify that the construct had been correctly inserted into appropriate selective plates by colony PCR verification, the protein expression was not able to be verified within the time constraints of the competition. We want to emphasize that this sequence may still be successful, but its efficiency was unable to be demonstrated within the time span that CCA_San_Diego had in the lab. For future teams, we advise testing construct designs with successful expression with a standard TEF promoter to compare expression levels, as was done in the initial study.

User Reviews

UNIQa3fb6f87e118afc4-partinfo-00000000-QINU UNIQa3fb6f87e118afc4-partinfo-00000001-QINU